Abstract: In this study,HPLC fingerprinting coupled with HPLC-3D chromatogram analysis was carried out to investigate the alkaloid components in Corydalis yanhusuo from 13 different regions.Cluster analysis was performed based on similarity and uncommon peaks gotten from 5 alkaloids.As the result,12 characteristic peaks were detected in samples from 13 regions,the similarity of their fingerprinting was higher than 0.9.At the same time,cluster analysis based on uncommon peaks showed that the samples can be clustered into three groups.Among them,samples from Bozhou Anhui,Guangxi Hechi and Liaoning Qingyuan were clustered one group,samples from Yunxi Hubei,Shandong Tonghua,and Heilongjian Suihua joined together in one group,while the last group contained the samples from Zhejiang Dongyang,Zhejiang Panan,Zhejiang Xianju and Hanzhong.The study indicated the components of C.yanhuso can be reflected by HPLC fingerprinting coupled with HPLC-3D chromatogram analysis,which was of great use in the quality control of this Chinese traditional medicine.

Key words: Corydalis yanhusuo, alkaloid, HPLC fingerprinting, cluster analysis