Abstract: The objective of this study was to determine the content of specnuezhenide in different habitat Fructus Ligustri Lucidum by near infrared spectroscopy.The near infrared spectroscopy was combined with partial least squares (PLS) analysis to establish the quantitative model of specnuezhenide in different habitat Fructus Ligustri Lucidum.The correlation coefficients (R²) of the quantitative calibration model for specnuezhenide was 0.98075,the root mean square error of calibration (RMSEC) was 0.216,the root mean square error of predication (RMSEP) was 0.223,the root mean square error of cross validation (RMSECV) was 0.52276.The developed method was convenient and accurate.It was feasible to determine the content of specnuezhenide in Fructus Ligustri Lucidum rapidly.

Key words: near-infrared spectroscopy, partial least squares, Fructus Ligustri Lucidum, specnuezhenide, content determination