This study aims to establish high-performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD) fingerprints and multi-component content determination method for Morindae Officinalis Radix (MOR) and processed products, and evaluate its quality in combination with chemometrics method. The oligosaccharide fingerprints of MOR and its processed products were analyzed by HPLC-ELSD and the content of eight oligosaccharide chemical components were determined. By means of similarity analysis, clustering analysis (HCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), the quality of MOR and different proccessed products was eveluated. The fingerprints of MOR and its proccessed products identified 15 common peaks, among which eight components were characterized. The similarity of fingerprints was greater than 0.90. MOR and different processed products are clustered together respectively by HCA and OPLS-DA. The four components, including peak 1(sucrose), peak 2(1-kestose), peak 3(nystose) and peak 4(1F-fructofuranosyl nystose), were the main makers of the difference between MOR and its processed products. The content determination results showed that there were signifficant differences in the content of eight oligosaccharides in MOR and processed products by different processing methods, in the order from high to low: salted MOR > prepared MOR > debarked MOR > MOR. This method is simple, feasible, stable and reliable, which can providing reference for the quality evaluation of MOR and its processed products.