天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (3): 538-545.doi: 10.16333/j.1001-6880.2026.3.009 cstr: 32307.14.1001-6880.2026.3.009

• 研究简报 • 上一篇    下一篇

青霉菌Penicillium glabrum SF-61290次级代谢产物及其生物活性研究

赵诗琴2，刘  芳1,3，王开梅1,3，方  伟1,3，刘曼莉1,3，龚  艳1,3，杨曦亮2*，张亚妮1,3*   

  1. 1湖北省农业科学院 湖北省生物农药工程研究中心，武汉 430064；2 武汉科技大学医学院药学系药物先进技术研究所 感染免疫与肿瘤微环境研究所 职业危害识别与控制湖北省重点实验室，武汉 430081；3国家植保微生物种质资源库（湖北），武汉 430064
  • 出版日期:2026-03-27 发布日期:2026-03-26
  • 基金资助:
    国家重点研发计划子课题（2021YFD1800402）；湖北省重点研发项目（2023BBB176）；农业微生物资源开发与利用（2024-620-000-001-023）

Secondary metabolites of Penicillium glabrum SF-61290 and their bioactivities

ZHAO Shi-qin2,LIU Fang1,3,WANG Kai-mei1,3,FANG Wei1,3, LIU Man-li1,3,GONG Yan1,3,YANG Xi-liang2 *,ZHANG Ya-ni1,3 *   

  1. 1Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Sciences,Wuhan 430064,China; 2 School of Medicine,Wuhan University of Science and Technology,Institute of Pharmaceutical Process,Institute of Infection,Immunology and Tumor Microenvironment,Hubei Province Key Laboratory of Occupational Hazard Identification and Control; 3National Plant Protection Microbial Germplasm Resource Bank (Hubei),Wuhan 430064,China
  • Online:2026-03-27 Published:2026-03-26

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摘要：

为明确青霉菌Penicillium glabrum SF-61290次级代谢产物及其生物活性，本研究采用硅胶柱色谱和制备高效液相色谱等方法，对其发酵液的乙酸乙酯提取物进行分离纯化，通过NMR、MS等现代波谱学方法鉴定化合物的结构；此外，分别采用MTT法和微量稀释法测定了化合物的细胞毒及抑菌活性。从该菌株发酵液的乙酸乙酯提取物分离到了10个化合物，分别鉴定为penicilazaphilone O（1）、核丛青霉素（2）、isochromophilone XV（3）、8a-epi-hypocrellone A（4）、arvoredol（5）、geumsanol G（6）、geumsanol D（7）、penicilazaphilone B（8）、geumsanol B（9）、isochromophilone VI（10）。其中，化合物1为新的阿扎菲酮衍生物。化合物3、5、7、10对结直肠癌HCT-8细胞表现出显著的细胞毒活性，其IC50值分别为13.1、27.84、17.96和54.17 μmol/L，优于阳性对照药物5-氟尿嘧啶（IC50 = 61.17 μmol/L）；化合物1~10对大肠杆菌、金黄色葡萄球菌、猪链球菌均无明显抑菌活性，其最小抑制浓度均大于100 μg/mL。

关键词: 青霉菌, 光孢青霉菌, 次级代谢产物, 阿扎菲酮, 生物活性

Abstract:

To characterize the secondary metabolites and evaluate their bioactivities of Penicillium glabrum SF-61290, the study was carried out to isolate and purify the ethyl acetate extract of its fermentation broth using silica gel column chromatography and preparative HPLC, and identify the structures of the compounds by modern spectroscopic methods such as NMR, MS, and so on. Additionally, the in vitro cytotoxic and antibacterial activities of these compounds were assessed using the MTT assay and the microdilution method, respectively. Ten compounds were isolated from the ethyl acetate extract of the strain fermentation broth, and identified as penicilazaphilone O (1), sclerotiorin (2), isochromophilone XV (3), 8a-epi-hypocrellone A (4), arvoredol (5), geumsanol G (6), geumsanol D (7), penicilazaphilone B (8), geumsanol B (9), and isochromophilone VI (10). Among these, compound 1 is a new azaphilone derivative. Compounds 3, 5, 7, and 10 exhibited significant cytotoxicity against the colorectal cancer HCT-8 cell line, with IC50 values of 13.1, 27.84, 17.96, and 54.17 μmol/L, respectively, outperforming the positive control 5-fluorouracil (IC50 = 61.17 μmol/L). Compounds 1-10 exhibited no obvious activities against Escherichia coli, Staphylococcus aureus, and Streptococcus suis, with minimal inhibitory concentration all exceeding 100 μg/mL.

Key words: Penicillium sp; Penicillium glabrum, secondary metabolites, azaphilones, bioactivities

中图分类号:  O629.9

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