紫球藻多糖化学结构解析

doi:A

天然产物研究与开发 ›› 2012, Vol. 24 ›› Issue (5): 569-574.doi: A

• 研究论文 • 下一篇

紫球藻多糖化学结构解析

孙利芹^1,2*，王凌¹，马翠华¹，王长海²

¹烟台大学生命科学学院，烟台 264005；²大连理工大学环境与生物工程系，大连 116024

收稿日期:2011-07-05 出版日期:2012-05-25 发布日期:2012-06-12
基金资助:
国家“十一五”科技支撑计划（2008BAD94B04）；山东省自然科学基金（Y2007D59; Y2008B21）；烟台市科技发展计划（2009219）

Analysis of Chemical Structures of Polysaccharide from Porphyridium Cruentum

SUN Li-qin^1,2*, WANG Ling¹, MA Cui-hua¹, WANG Chang-hai²

¹Life science college of Yantai University, Yantai 264005, China; ²Bioscience and Engineering department of Dalian university of Technology, Dalian 116024, China

Received:2011-07-05 Online:2012-05-25 Published:2012-06-12

摘要/Abstract

摘要： 为明确紫球藻多糖的化学结构，本文采用化学分析和光谱分析方法对紫球藻多糖的一级糖链结构进行了分析。GC分析表明该多糖由木糖、葡萄糖和半乳糖组成，为一种杂多糖，其摩尔比为：2.96：1.25：3.06；红外光谱分析结果显示紫球藻多糖为硫酸化多糖，糖苷键类型为β构型；化学分析结果推断紫球藻多糖糖链连接方式以1→3为主，存在少量1→2，1→4，1→6键型，且半乳糖在支链或主链末端有较大量的存在，木糖和葡萄糖在主链或靠近主链区域有特定分布；NMR分析显示紫球藻多糖的硫酸酯基连在C-6上，且多糖的糖苷键为β型；GC-MS联机分析进一步确定紫球藻多糖为一种主要含有1→3糖苷键，并含有1→4，1→6糖苷键的杂多糖。综合上述分析，推断出紫球藻多糖的糖链主链的重复单元结构。

关键词: 紫球藻, 多糖, 糖链结构, 化学分析, 光谱分析

Abstract: In this paper, the chemical analysis and spectrum analysis methods were used for investigating the level 1 sugar chain structure of Porphyridium cruentum polysaccharide (EPS). The results of GC analysis showed that the sugar moiety of this polymer EPS was composed of three neutral monosaccharides (xyl, glc and gal), and molar ratio was 2.96:1.25:3.06. Infrared spectrum analysis showed that EPS was sulfuric acid ester polysaccharide, and its absolute configuration was β. The results of chemical analysis revealed that the main linkage of EPS was β-(1→3), and there were a few of 1→4 and 1→6 linkages. A large of galactoses exsited at the end or branch of sugar chain, but xylose and glucose were distributed specially close to main chain. NMR analysis displayed that sulfuric acid ester linked on C-6, and glycosidic bond was β-configuration. GC-MS on-line analysis further confirmed that EPS was a heteropolysaccharide, and sugar chains structure mainly contained 1→3 linkage and small number of 1→4, 1→6 linkage. Based on above comprehensive analysis, the repeat units of polysaccharide produced by P. cruentum could be inferred as D-Xyl and D-Glc, which were present at the main chain with β-(1→3, 1→4) linkage and D-Gal mainly existed at the end of main chain and branched chain.

Key words: Porphyridium cruentum, polysaccharide, sugar-chain structure, chemical analysis, spectrographic analysis

中图分类号:

Q539

孙利芹，王凌，马翠华，王长海. 紫球藻多糖化学结构解析[J]. 天然产物研究与开发, 2012, 24(5): 569-574.

SUN Li-qin, WANG Ling, MA Cui-hua, WANG Chang-hai. Analysis of Chemical Structures of Polysaccharide from Porphyridium Cruentum[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 24(5): 569-574.

参考文献 1

Zhu GJ(诸葛健),Zhao ZF(赵振锋).Fang HY(方慧英).The relationship between structure and function of polysaccharides.J Wuxi Univ Light Ind (无锡轻工大学学报),2002,21:209-212.2 Sheng SQ,Chermiak R.Structure of the capsular polysaccharide of Clostridium perfingens Hobbs 10 determined by NMR spectroscopy.Carbohydr Res,1998,305:65-72.3 Guo ZC(郭振楚).Sugar chemistry(糖类化学)Beijing:Chemistry Industry Press,2005.4 Gloaguen V,Ruiz G,Morvant H,et al.The extracellular polysaccharide of Porphyridium sp:an NMR study of lithium-resistant oligosaccharidic fragments.Carbohydr Res,2004,339:97-103.5 Geresh S,Adin I,Yarmolinsky E,et al.Characterization of the extracellular polysaccharide of Porphyridium sp.:molecular weight determination and rheological properties.Carbohydr Polym,2002,50:183-189.6 Huheihel M,Ishanu V,Tal J,et al.Activity of Porphyridium sp.Polysaccharide against herpes simples viruses in vitro and in vivo J Biochem Bioph methods,2002,50:189-200.7 Sun LQ,Wang CH,Shi QJ,et al.Preparation of different molecular weight polysaccharides from Porphyridium cruentum and their antioxidant activities.Int J Biol Macromol,2009,(45):42-47.8 Irit D,Renven C,UrielSod M.et al.Solube polysaccharide and biomass of red microalga Porphyridium sp alter intestinal morphology and reduce serum cholesterol in rats.British J Mutri,2000,184:469-472.9 Gu NY(顾宁琰),Liu YF(刘宇峰).Antiirradiation activities of extracellular polysaccharide of Porphyridium sp.Mar Sci (海洋科学),2002,26(12):53-56.10 Zhu GF(朱桂芳),Wei D(魏东),Liu SS(刘石生),et al.Seperation,Purif ication and Property of Extracellular Polysaccharide from Porphyridium sp.Nat Prod Res Dev(天然产物研究与开发),2005,17:542-548.11 Zhang WJ(张惟杰).Study on Biochemistry Technology of Glycoconjugates(糖复合物生化研究技术),2st Ed.Hangzhou:Zhejiang University Press,2003,36-242.12 Liu CH(刘纯慧),Ye L(叶亮),Lin QX(林亲雄),et al.Studies on preparation and antitumor activity of the polysaccharide from the eggs of Strongylocentrotus nudus (SEP).Pharm Biotechnol(药物生物技术),2006,13:429-432

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紫球藻多糖化学结构解析

Analysis of Chemical Structures of Polysaccharide from Porphyridium Cruentum

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