天然产物研究与开发 ›› 2013, Vol. 25 ›› Issue (12): 1627-1630.

• 研究论文 • 上一篇    下一篇

一株果胶酶产生菌的筛选、鉴定及其产酶条件研究

袁志辉*   

  1. 湖南科技学院生命科学与化学工程系,永州 425100
  • 出版日期:2013-12-31 发布日期:2015-01-08

Screening and Identification of Pectinase Producing Strain and Study on Its Pectinaseproducing Conditions

YUAN Zhi-hui*   

  1. Hunan University of Science and Engineering,Hunan Yongzhou 425100,China
  • Online:2013-12-31 Published:2015-01-08

摘要: 为从自然界寻找高效的果胶酶生产菌,以用于后续的研究和应用,采集富含果胶质的土壤样品,从中筛选分离出11株产果胶酶菌株。以其中一株产酶活力最高的菌株HYL6为研究对象,对其进行了种属鉴定并初步探索了最佳的产酶条件。菌株鉴定结果表明,菌株HYL6为芽孢杆菌属。产酶条件优化及正交试验表明,菌株HYL6最佳的产酶条件组合为:可溶性淀粉5 g/L,蛋白胨15 g/L,pH值7时,36 ℃、180 rpm振荡培养34 h。在这一条件下进行发酵,最高产酶活力可达415 U/mL。

关键词: 果胶酶, 酶活力, 产酶条件

Abstract: The aim of this work was to screen highly pectinase producing bacteria for following laboratorial research and industrial application.The soil sample that is rich in pectin substance was collected from ramie field located in the suburbs of LingLing district for screening of pectinase producing bacteria,and 11 stains were gained by enrichment procedure and screening test successively.Strain HYL6 which produced the highest enzyme activity tested by 3,5dinitrosalicylic acid (DNS) method in 11 strains was identified as Bacillus sp.and its pectinase producing conditions were optimized by onefactor experiments and orthogonal test successively.The optimized conditions were as follows:soluble starch 5 g/L,peptone 15 g/L,pH 7,and shaking fermentation 34 hours at 36 ℃,180 rpm.The activity of pectinase produced by the strain HYL6 under the optimal conditions reached 415 U/mL.

Key words: pectinase, enzyme activity, pectinase-producing conditions

中图分类号: 

Q939.96