关键词: 野生蕨麻, 人工栽培蕨麻, HPLC, 蕨麻苷, 指纹图谱

Abstract: To analyze the difference of the content of anserinoside and fingerprints between the wild and the artificial cultivated Potentilla anserina No.1.Samples were extracted with 70% ethanol and purified with D101 macroporous adsorption resin to enrich its composition.The extract solutions were evaporated to dry and the residue was dissolved with methanol.The chromatographic system consisted of an Kromasil 100-5-C₁₈ and mobile phase of methanol:water (60:40)with the detector set at 208 nm in room temperature.The flow rate of 1.0 mL/min.Acetonitrile in water was used as mobile phase for gradient linear eluting to get the fingerprint,other conditions is the same as the first time.The peak area of anserinoside and its filling quantity within 0.5~5 μg had good linear relationship.The regression equation is (A=381.41C-11.75,R=0.9994,n=6),the average recovery was 97.7%(n=6,RSD=3.0%).The content of anserinoside in the artificial cultivated Potentilla anserina No 1 have reached or exceeded those in the wild one.Fingerprint matching,similarity degree has reached to 84.5%.It is preliminarily feasible for the artificial cultivated potentilla anserina No 1 used as herbal medicine to replace the wild one.

Key words: wild Potentilla anserine, cultivated Potentilla anserine, HPLC, anserinoside, fingerprints