天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 124-129.doi: 10.16333/j.1001-6880.2020.S.020

• 开发研究 • 上一篇    下一篇

响应面法优化酶解碱提沙棘籽粕蛋白的工艺研究

王继飞1,4，欧阳健2，胡娜1,3，王洪伦1,2,3*   

  1. 1中国科学院西北高原生物研究所 中国科学院藏药研究重点实验室，西宁 810008；2中国科学院西北高原生物研究所 湖州高原生物资源产业化创新中心，湖州 313000；3青海省藏药研究重点实验室，西宁 810008；4中国科学院大学，北京 100049

  • 出版日期:2020-06-28 发布日期:2020-07-21
  • 基金资助:
    青海省科技厅项目（2017-SF-A8，2017-ZJ-Y11，2019-ZJ-7023）

Optimization of protein extraction process from Hippophae rhamnoides seed meal by response surface methodology

WANG Ji-fei1,4,OUYANG Jian2,HU Na1,3,WANG Hong-lun1,2,3*   

  1. 1Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;2Huzhou plateau biological resource center of innovation,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Huzhou 313000,China;3Key Laboratory of Tibetan Medicine Research of Qinghai Province,Xining 810008,China;4University of the Chinese Academy of Sciences,Beijing 100049,China

  • Online:2020-06-28 Published:2020-07-21

PDF (PC)

110

摘要： 本文以沙棘籽粕为原料，采用醇提法预处理材料，以酶解碱提联用法提取蛋白粗提物，通过单因素实验，对影响沙棘籽粕蛋白提取得率的液料比、温度、pH、胰蛋白酶加酶量、提取时间、提取次数等因素进行筛选和初步优化，通过基于Box-Behnken Design的响应面法研究最佳提取工艺，验证响应面优化结果。分析响应面结果，沙棘籽粕蛋白提取的最优工艺参数为：液料比44∶1（mL∶g）、pH12.3、胰蛋白酶加酶量1.4%、温度60 ℃、提取时间30 min、提取次数3次。此条件下，蛋白实际提取得率50.53%，接近数学模型的预测值51.54%。


关键词: 沙棘籽粕蛋白, 响应面法, 酶解碱提, 工艺优化

Abstract:

In this study,the protein was extracted by enzymatic hydrolysis alkali solubilization from the Hippophae rhamnoides seed meal prepared by alcohol extraction.Through the single factor experiments,the factors affecting the protein extraction yield were screened and preliminaries optimized.The optimum conditions of liquid-to-solid ratio,pH and trypsin amount were obtained via response surface of Box-Behnken design.Experimental results showed the optimal protein extraction conditions are liquid-to-solid ratio 44∶1（mL∶g）,temperature 60 ℃,pH12.3,trypsin amount 1.4%,extraction time 30 min and 3 times.The validation experiment demonstrated optimization results.Under the conditions,the actual yield of protein was 50.53%,closed to predicted value 51.54% by model.

Key words: Hippophae rhamnoides , seed meal protein, response surface, enzymatic hydrolysis alkali solubilization, process optimization

中图分类号:  TS255.36

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：