天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (2): 227-235.doi: 10.16333/j.1001-6880.2021.2.007

• 研究简报 • 上一篇    下一篇

贮存年份对北艾和蕲艾精油成分与抑菌活性的影响研究

蒋志惠1,郑婧龙1,谷令彪1,李志祥2,刘运革2,马立静1,王敏1,申艳红1*   

  1. 1安阳工学院 河南省兽用生物制品研发与应用国际联合实验室;2河南省亚临界生物技术有限公司,安阳 455000
  • 出版日期:2021-02-28 发布日期:2021-03-03
  • 基金资助:
    河南省重点研发与推广专项(科技攻关)(182102310 686);河南省科技攻关重点项目(192102110102)

Effect of storage years on the chemical components and antibacterial activity of essential oils in Artemisia argyi (Beiai and Qiai)

JIANG Zhi-hui1,ZHENG Jing-long1,GU Ling-biao1,LI Zhi-xiang2,LIU Yun-ge2,MA Li-jing1,WANG Min1,SHEN Yan-hong1*   

  1. 1Henan Joint International Research Laboratory of Veterinary Biologics Research and Application,Anyang Institute of Technology;
    2Henan Subcritical Extraction Biological Technology Co.,Ltd,Anyang 455000,China

  • Online:2021-02-28 Published:2021-03-03

摘要: 本文采用水蒸汽蒸馏法提取了贮存0、1、2年的北艾和蕲艾精油,采用GC-MS检测精油化学成分,选取10种常见细菌,检测了其抗菌谱和最小杀菌浓度。结果发现:0、1年份北艾精油中小分子挥发性物质较多,随着贮存年份的增加,大分子挥发性物质随之增加;侧柏酮为蕲艾的特有成分。0年份艾叶精油的抑菌活性较高,对沙门菌、枯草芽孢杆菌、沙门菌耐药菌、绿脓杆菌均具有抑制作用。综上,不同贮存年份和品种的艾叶精油在化学成分、抗菌谱和抑菌活性方面均存在差异,综合考虑精油含量和抑菌活性,以0年份的北艾为原料提取精油最佳。

关键词: 艾叶, 精油, 化学成分, 抑菌活性

Abstract:

The effects of different storage years on chemical components and the antibacterial activities of essential oils in Artemisia argyi(Beiai and Qiai) were studied to provide data references for screening the better antimicrobial activity of Artemisia.The essential oils of Beiai and Qiai in 0,1st,and 2nd years were extracted by water distillation.The chemical compositions of essential oils were identified by GC-MS.To study the antibacterial activity of the essential oils,10 kinds of common bacteria were selected,and the antibacterial spectrum and minimum bactericidal concentration of the essential oils were detected.The results showed that storage years affect the chemical compositions and bacteriostasis activity of the essential oils of Beiai and Qiai.The GC-MS and compounds analysis results showed that the small molecules of volatile substances were higher in the 0 and 1st years of Beiai than others.With the increase of storage years,the content of macromolecular volatile substances increased.Compared with Beiai,thujone was found only in Qiai.The results of the antimicrobial spectrum showed that the essential oil of the Artemisia argyi family has a wide antimicrobial spectrum,but there are differences.Among the different storage years,the antibacterial activity of the Artemisia argyi family in year 0 was best among them.The essential oil of Beiai and Qiai has better antibacterial effects on SalmonellaBacillus subtilisSalmonella-resistant bacteria and Pseudomona aeruginosa.A comprehensive analysis of essential oil content and antibacterial activity showed that the essential oil of Beiai in year 0 was the best choice.

Key words: Artemisia argyi, essential oil, chemical composition, antibacterial activity

中图分类号:  O659.12