天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (4): 592-597.doi: 10.16333/j.1001-6880.2021.4.008

• 研究简报 • 上一篇    下一篇

紫茎泽兰中泽兰酮类成分的抗炎活性研究

孙丽芳1,2，周雪2,3，汪杰2,3，李海池2,3，葛发欢2,3*   

  1. 1广东药科大学中药学院，广州510006；2广东省中药超临界流体萃取工程技术研究中心，广州511458；3中山大学药学院，广州510006

  • 出版日期:2021-04-28 发布日期:2021-05-10
  • 基金资助:
    国家重点研发计划（2017YFC1703102）；广东省重点领域研发计划（2020B1111110003）

Study on the anti-inflammatory activity of euptox A and 9-oxoageraphorone in Eupatorium adenophorum Spreng#br#
#br#

SUN Li-fang1,2,ZHOU Xue2,3,WANG Jie2,3,LI Hai-chi2,3,GE Fa-huan2,3*   

  1. 1School of Tiaditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China; 2Guangdong Research Center for Supercritical Fluid Extraction of Chinese Medicine,Guangzhou 511458,China; 3School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China

  • Online:2021-04-28 Published:2021-05-10

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摘要：

探讨紫茎泽兰提取物与单体的体外抗炎活性，为寻找紫茎泽兰的抗炎有效成分提供依据。以超临界CO2提取物（SFE）、超临界CO2萃取-分子蒸馏提取物（MDE）、甲醇提取物（MSE）及泽兰酮单体9-羰基-10,11-去氢泽兰酮(euptoxA)、9-羰基-10-Hβ-泽兰酮（9-oxoageraphorone）为实验材料，采用体外脂多糖（LPS）诱导小鼠单核巨噬细胞RAW264.7炎症模型，通过对NO以及TNF-α炎症因子的测定，进行紫茎泽兰各提取部位及泽兰酮单体的抗炎活性研究。超临界CO2提取物、超临界CO2萃取-分子蒸馏提取物、甲醇提取物及单体euptox A、9-oxoageraphorone均具有抗炎活性；泽兰酮单体euptox A、9-oxoageraphorone均具有显著的抗炎活性，抗炎活性高于各个提取物，其中9-oxoageraphorone的NO抑制率（IC50 = 10.09 μg/mL）大于euptox A（IC50 = 16.23 μg/mL）。本实验发现富含泽兰酮类成分的提取物及泽兰酮单体有较好的抗炎活性，泽兰酮类成分是紫茎泽兰的发挥抗炎作用的有效成分之一，为紫茎泽兰的资源开发与利用提供了依据。

关键词: 茎泽兰；提取物, euptox A, 9-oxoageraphorone, 抗炎活性

Abstract:

In this study,the in vitro anti-inflammatory activities of several extracts or purified monomers from Eupatorium adenophorum Spreng were investigated in order to find the major anti-inflammatory active ingredients of E. adenophorum.The extracts were obtained using supercritical fluid extraction CO2-(SFE),supercritical CO2 extraction-molecular distillation-(MDE) and methanol extract-(MSE),separately,and the tested monomers were euptox A and 9-oxoageraphorone.In vitro lipopolysaccharide (LPS)-induced RAW264.7 inflammation model of mouse mononuclear macrophages was used and NO and TNF-α inflammatory factors were measured to study the anti-inflammatory activities.All extracts and monomers showed well anti-inflammatory activities,but the inflammatory activity of the two monomers were higher than those of extracts.The NO inhibition rate of 9-oxoageraphorone (IC50=10.09 μg/mL) was higher than that of euptox A (IC50 = 16.23 μg/mL).It was found that extracts rich in euptox A and 9-oxoageraphorone had good anti-inflammatory activities,as well as the purified monomers,indicating that euptox A and 9-oxoageraphorone were the effective anti-inflammatory components of E. adenophorum.This article provided a basis for the resource development and utilization of E. adenophorum.

Key words: Eupatorium adenophorum Spreng, extract, euptox A, 9-oxoageraphorone, anti-inflammatory activity

中图分类号:  Q942.6 R285.5

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