天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (7): 1165-1171.doi: 10.16333/j.1001-6880.2021.7.011

• 研究简报 • 上一篇    下一篇

深海真菌Neoroussoella sp.固体发酵物中次生代谢产物及其生物活性研究

钟晋谦1,2,陈玉婵2,陈闪冲2,刘昭明2,刘洪新2,章卫民2*,严寒静1 *    

  1. 1广东药科大学中药学院,广州 510006;2广东省科学院微生物研究所 华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室,广州 510070

  • 出版日期:2021-07-28 发布日期:2021-07-29
  • 基金资助:
    广东省海洋经济发展专项([2020]042);广东省自然科学基金团队项目(2016A030312014);国家自然科学基金(41906106);广东省特支计划(2019TQ05Y375)

Study on the secondary metabolites from the deep-sea-derived fungus Neoroussoella sp.and their biological activities 

ZHONG Jin-qian1,2,CHEN Yu-chan2,CHEN Shan-chong2,LIU Zhao-ming2,LIU Hong-xin2,ZHANG Wei-min2*,YAN Han-jing1*   

  1. 1School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;2 Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences, Guangzhou 510070,China

  • Online:2021-07-28 Published:2021-07-29

摘要: 研究深海真菌Neoroussoella sp.FS526的次生代谢产物及生物活性。采用硅胶柱、Sephadex LH-20凝胶柱和高效液相等多种色谱技术对菌株FS526的固体发酵物中的次生代谢产物进行分离纯化,并通过NMR等波谱方法鉴定化合物结构。从菌株FS526中共分离鉴定了15个化合物,分别为amestolkolide B(1)、thailandolide B(2)、aspergillumarins A和B(34)、de-O-methyldiaporthin(5)、4-(2-acetoxyethyl)phenol(6)、5,6,8-三羟基-4-(1′-羟乙基)异香豆素(7)、sescandelin(8)、penicifuran A(9)、7-羟基-5-甲氧基-4,6-邻苯二甲酯(10)、pestalotiolactone A(11)、2′,4′-二羟基-6′-甲氧基-3′-甲基苯乙酮(12)、1-(2,4-二羟基-6-甲氧基-3-甲基苯基)乙酮(13)、2,5-二甲基间苯二酚(14)、3,5-二羟基甲苯(15)。所有化合物均为首次从该属真菌中分离得到。细胞毒和抗氧化活性测试表明化合物1具有较弱的细胞毒活性,其IC50值为41.44~77.89 μM;化合物7具有较强的抗氧化活性,其IC50值为13.93 μM。


关键词: 深海真菌, Neoroussoella sp., 次生代谢产物, 细胞毒活性, 抗氧化活性

Abstract:

The secondary metabolites of a deep-sea-derived fungus Neoroussoella sp. FS526 and their biological activities were investigated.The solid fermentation extraction of strain FS526 was isolated and purified by silica gel column,Sephadex LH-20 gel column,and high performance liquid chromatography,which led to the isolation of fifteen known compounds.The chemical structures of the isolates were identified by spectrum analysis as amestolkolide B (1),thailandolide B (2),aspergillumarins A-B (3 and 4),de-O-methyldiaporthin (5),4-(2-acetoxyethyl)phenol (6),5,6,8-trihydroxy-4-(1′-hydroxyethyl)isocoumarin (7),sescandelin (8),penicifuran A (9),7-hydroxy-5-methoxy-4,6-dimethylphthalidc (10),pestalotiolactone A (11),2′,4′-dihydroxy-6′-methoxy-3′-methylacetophenone (12),1-(2,4-dihydroxy-6-methoxy-3-methylphenyl)ethanone (13),2,5-dimethylresorcinol (14),and 3,5-dihydroxytoluene (15).All the isolated compounds were firstly reported from the genus Neoroussoella.Compound 1 showed weak cytotoxic activity with the IC50 values of 41.44-77.89 μM,and compound 7 showed strong antioxidant activity with the IC50 value of 13.93 μM.

Key words: deep-sea-derived fungus, Neoroussoella , sp., secondary metabolites, cytotoxic activity, antioxidant activity

中图分类号:  R914.4