In this study,an endophytic strain HJ-3 was screened from Polygonatum cyrtonema,an original medicinal material from Anhui Province.The antagonistic effects of HJ-3 on eight plant pathogenic fungi were observed by five-point confrontation culture method.The activated HJ-3 strain was fermented into fermentation products with different concentrations,its antibacterial activities against four tested bacteria were detected by filter paper diffusion method,and the extracted fermentation products were detected by LC-MS.The results showed that the endophytic bacteria HJ-3 showed obvious antagonistic activities against eight common plant pathogenic fungi,among which the antagonistic activities against seven pathogenic fungi such as Rhizoctonia solani Kühn were obvious,and the antagonistic activities against Collettrichum gloeosporioides Penz was weak.In addition,it was found that the fermentation products of different concentrations had certain antibacterial effects on the four kinds of tested bacteria,among which the antibacterial effects on Staphylococcus aureus and Bacillus subtilis were better.The endophytic strain HJ-3 was identified and named as Bacillus velezensis by morphological analysis and 16S rRNA gene sequencing.A 5,5′-methylenebis(2-tert-butyl-4-methylphenol) phenolic substance and three similar compounds were obtained by LC-MS analysis.The results of this study laid a foundation for the development of endophytic bacteria from Polygonatum cyrtonema,it also provides the basis for the extensive application of endophytic drugs.