This study investigated the anti-hypoxia effect and potential targets of American ginseng using zebrafish model and dynamic molecular docking technique.Zebrafish of the AB line were used as experimental animals,and anhydrous sodium sulfate was used as a modeling agent to induce hypoxia in juvenile zebrafish,and the anti-hypoxia effects of American ginseng were comprehensively evaluated.The active ingredients and hypoxia-related common targets of American ginseng were screened by the network pharmacology technique,and the protein-protein interaction network was constructed by STRING platform and Cytoscape 3.8.2 software to find the potential targets of the anti-hypoxia effect of American ginseng,and the binding ability and stability of the active ingredients to the key targets were verified by dynamic molecular docking technique.In vivoexperiments on zebrafish showed that the extract of American ginseng significantly increased the survival rate under hypoxic conditions and reduced the neurobehavioral state caused by hypoxia (P < 0.05),and showed a dose-dependent correlation.Dynamic molecular docking results showed that total seven potential active compounds had good spontaneous binding ability with five potential anti-hypoxic targets,among which TNF,HSP90AA1 might be the key potential targets of American ginseng against hypoxia.In summary,The anti-hypoxia model of zebrafish larvae established in this experiment can quickly and easily evaluate the anti-hypoxia activity of American ginseng samples,and the results of dynamic molecular docking showed that the effect may work through TNF and HSP90AA1 targets,which provides a reference basis for the subsequent study of the anti-hypoxia mechanism of American ginseng.