This study aims to study the immunomodulatory effect of Hedysari Radix water extract freeze-dried powder on mouse monocyte macrophages (RAW 264.7 cells) before and after "rubbing strips" and its possible mechanism of action.Using CCK8 method to detect the effect of different concentrations of Hedysari Radix on the proliferation ability of RAW 264.7 cells.Using neutral red phagocytosis,Griess assay and ELISA method to detection the the phagocytic capacity,NO and tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β).The secretion of interleukin-6(L-6).Determination of phosphorylation expression of major nuclear factor pathway proteins p65 and MAPKs pathway proteins p38 and JNK in macrophages by Western-blot.The results indicate that mass concentration of Hedysari Radix water extract freeze-dried powder was between 3.125-800 μg/mL,it has a positive promoting effect on macrophage proliferation,and at 200 μg/mL has a significant impact on the value-added rate (P<0.01).In addition,Hedysari Radix is found in the range of 50-200 μg/mL,it can significantly enhance the phagocytic activity,NO release,and the secretion of TNF-α,IL-1β,IL-6 was dose-dependent (P<0.05),and the effect of rubbing Hedysari Radix was better than that of unrubbing Hedysari Radix.Western blot results showed that compared with the blank group,Hedysari Radix could increase the phosphorylation levels of p38,JNK,and p65 proteins in RAW 264.7 cells,suggesting that Hedysari Radix may activate NF-κB and MAPKs signaling pathways play an immunomodulatory role.From this,it can be concluded that the processing of rubbing from the place of origin may enhance its immune regulatory effect.The research results obtained from the experiment can provide scientific theoretical support for the identification of the quality of initial processing in the origin of Hedysari Radix.

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