关键词: 金银花, 绿原酸, 木犀草苷, HPLC, 隶属函数, 海螺沟

Abstract: Lonicera japonica Thumb.is one of the Chinese traditional medicinal herbs.In recent year,it have great demand in medicine,spices,cosmetics and health food,with high medicinal value and economic value.In order to explore the suitable planted varieties of Lonicera japonica in the Hailuogou scenic area,the introduction experiment was conducted on 21 main cultivars obtained from Shangdong,Henan,Hebei and Sichuan province.The high performance liquid chromatography(HPLC)was used to determine the content of chlorogenic acid and galuteolin in different kinds of L.japonica. The results were as follows:1) The contents of chlorogenic acid in the L.japonica flos were 1.617%-3.394%,higher than specified value(1.5%) in Chinese Pharmacopoeia.They were 0.613%-1.712% in the stem and 1.257%-2.475% in the folium.The contents of galuteolin in the flos were 0.009%-0.184%.The contents of galuteolin in Zhongjin 1 and Mengjin 1 obtained from Shangdong were 0.009% and 0.029%,which were lower than the specified value(0.050%) in Chinese Pharmacopoeia.They were 0.017%-0.076% in the stem and 0.406%-0.562% in the folium.2) The subordinate function of comprehensive evaluation of chlorogenic acid and gluteolin contents in the flos,stem and folium showed that Lufengwang 3 obtained from Shangdong was suitable for planting in the Hailuogou scenic area.

Key words: Lonicera japonica Thumb., chlorogenic acid, galuteolin, HPLC, subordinate function, Hailuogou