关键词: 丹参, 毛状根, 诱导效应, 不同品系

Abstract: The quality of Salvia miltiorrhiza is different in different genetic backgrounds;however,the induction effect of Salvia miltiorrhiza hairy root has not been verified.In this experiment,transformed hairy roots of seven different Salvia miltiorrhiza strains were obtained by the infection of  Agrobacterium rhizogenes C58C1 and a hairy root culture system in vitro was established.By comparing induction efficiency,growth rate,content of active component from the above seven different genetic background Salvia miltiorrhiza's hairy root,the results showed that the induction effects of hairy root in different genetic backgrounds were different.Among them,the induction rate of Ds2 strain was lowest,which was significantly lower than that of the other 6 lines;the growth rates of three strains of Ds5,Ds6 and Ds7 were lower than Ds1,Ds3 and Ds4 strains;comparing the effective components content of three strains of Ds1,Ds3 and Ds4,it was found that the differences were obvious,and each had its advantages.Therefore,it was the first time to verify the induction effect of hairy root of Salvia miltiorrhiza in different genetic backgrounds.At the same time,through comprehensive evaluation,we screened three fine Salvia miltiorrhiza strains Ds1,Ds3 and Ds4 which were suitable for efficient fermentation of hairy roots.Rosmarinic acid was produced by Ds1 and Ds4,salvianolic acid B was produced by Ds4,and Ds3 was used to produce tanshinoneⅠ,Ds3 was used to produce tanshinoneⅡA.

Key words: Salvia miltiorrhiza, hairy root, inductive effect, different strains