天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (2): 256-267.doi: 10.16333/j.1001-6880.2021.2.010

• 开发研究 • 上一篇    下一篇

甘草来源内生真菌多样性和抗菌活性研究

刘文杰,李陇强,唐佳慧,姜淑玲,杨平,陈春晨,郭文强*   

  1. 井冈山大学医学部,吉安 343009
  • 出版日期:2021-02-28 发布日期:2021-03-04
  • 基金资助:
    井冈山大学自然科学科研项目(JZ1803);江西省教育厅科技计划(GJJ180558)

Study on the diversity and antibacterial activity of endophytic fungi from Glycyrrhiza uralensis 

LIU Wen-jie,LI Long-qiang,TANG Jia-hui,JIANG Shu-ling,YANG Ping,CHEN Chun-chen,GUO Wen-qiang*   

  1. Department of Medicine,Jinggangshan University,Ji’an 343009,China
  • Online:2021-02-28 Published:2021-03-04

摘要:

甘草作为使用广泛的根茎入药中药材,其自身复杂的微环境蕴藏了丰富的微生物资源,是未被充分发掘的新结构、新机制抗菌活性天然产物资源库。本文主要聚焦内蒙、甘肃和宁夏甘草中内生真菌,经过表观形态学特征分析,结合ITS-rDNA共获得134株真菌,其归属于16个属,显示出较好的真菌群落多样性,同时还发现青霉和曲霉属为各地甘草内生真菌的优势菌属。对所得内生真菌静置发酵后获得的代谢粗提物,利用纸片扩散法进行抗菌活性筛选与分析,发现其中35株真菌次级代谢产物具有广谱的抗菌活性。对比HPLC-UV指纹图谱分析结果,发现多个内生真菌的次级代谢产物中具有系列紫外吸收的天然产物。本研究表明,不同产地的甘草来源内生真菌群落具有丰富的多样性和良好的抗菌活性,预示着潜在的新颖抗菌天然产物研究价值。

关键词: 甘草, 内生真菌, 多样性, 抗菌活性

Abstract:

As widely used Chinese medicinal material,G. uralensis had complex endogenous microenvironment,which contained rich microbial resources.It was a treasure of antibacterial natural products that had not been fully explored,which was a potential resource for new structures and mechanisms of antibiotics.This article focuses on the diversity and antimicrobial activity of endophytic fungi from G. uralensis in Inner Mongolia,Gansu and Ningxia.After being dereplicated by morphological characteristics,134 fungi were identified by ITS-rDNA sequencing.We confirmed that these isolates belong to 16 different genera,which showed great fungal diversity,and Penicillium and Aspergillus were the dominant endophytic fungi in G. uralensis. Using classic fermentation condition,the crude extracts were screened for antibacterial activity by the paper diffusion method,and 35 strains of fungal secondary metabolites had broad-spectrum antibacterial activity.Comparing the results of HPLC-UV fingerprint analysis,many secondary metabolites of endophytic fungi had series of natural products with analogous ultraviolet absorption.Studies had demonstrated that endophytic fungi from the G. uralensis had rich diversity and superior antibacterial activity,which showed the potential for novel antibacterial natural products.

Key words: Glycyrrhiza uralensis,endophytic fungi,diversity,antibacterial activity 

中图分类号:  R915