In order to investigate the effect of wogonin on atherosclerosis and arterial inflammation in ApoE^-/- mice fed with high fat diet,ApoE^-/- mice were divided into normal diet group (ND),high-fat diet group (HFD),high-fat diet + wogonin (10 mg/kg) group (HFD + wogonin 10 mg/kg) and high-fat diet + wogonin (20 mg/kg) group (HFD + wogonin 20 mg/kg).Mice were given high-fat diet and wogonin for 12 weeks and then the arterial tissues were collected.RT-qPCR was used to detect the mRNA expression of IL-6,TNF-α,ICAM-1 and VCAM-1.Immunofluorescence staining was used to detect the expression of Moma-2 and VCAM-1 in the aortic root.Oil red O staining and HE staining were used to detect the changes of the lipid deposition and pathological changes in the aortic root.Biochemical kits were used to detect the serum lipid levels.Western blot was used to detect the phosphorylation of NF-κB and the expression of IκB-α.The results showed that compared with HFD group,the area of atherosclerotic plaque in HFD+wogonin group was decreased (P<0.05),but the serum lipid levels do not change; moreover,monocyte/monocyte macrophage infiltration,the protein expression of VCAM-1,the mRNA expression level of TNF-α,IL-6,ICAM-1 and VCAM-1 and the activation of NF-κB signal pathway in HFD+wogonin group were significantly reduced (P<0.05).These findings indicated that wogonin could effectively alleviate arterial atherosclerosis and inhibit NF-κB-mediated arterial inflammation in ApoE^-/- mice induced by high-fat diet.