天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (7): 1112-1120.doi: 10.16333/j.1001-6880.2021.7.005

• 研究论文 • 上一篇    下一篇

艾纳香地上部分化学成分及其抗氧化与酪氨酸酶抑制活性研究

周立强1,2,3,熊燕1,2,5,陈俊磊1,2,张嘉瑜1,2,郝小江1,2,4*,顾玮1,2*   

  1. 1贵州医科大学 省部共建药用植物功效与利用国家重点实验室;2贵州省中国科学院天然产物化学重点实验室,贵阳 550014;3贵州医科大学 药学院,贵阳 550025;4中国科学院昆明植物研究所,昆明 650201;5贵阳护理职业技术学院,贵阳 550081

  • 出版日期:2021-07-28 发布日期:2021-07-29
  • 基金资助:
    贵州省科技计划(黔科合支撑[2018]2799);国家自然科学基金(U1812403,31860074)

Study on the chemical constituents from the aerial parts of Blumea balsamifera DC. and their antioxidant and tyrosinase inhibitory activities

ZHOU Li-qiang1,2,3,XIONG Yan1,2,5,CHEN Jun-lei1,2,ZHANG Jia-yu1,2,HAO Xiao-jiang1,2,4*,GU Wei1,2*   

  1. 1State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;2The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;3Pharmacy School of Guizhou Medical University,Guiyang 550025,China;4Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;5Guiyang vocational and Technical College of Nursing,Guiyang 550081,China

  • Online:2021-07-28 Published:2021-07-29

摘要:

本文对艾纳香(Blumea balsamifera DC.)中的化学成分及其抗氧化与酪氨酸酶抑制活性进行了研究。利用各种色谱手段和波谱学方法,从艾纳香地上部分分离得到18个化合物,分别鉴定为:7,3′,4′-三甲基槲皮素(1)、4′,5-二羟基-3′,7-二甲氧基黄酮(2)、木犀草素-7-甲醚(3)、鼠李素(4)、(2R,3R)-3,3′,5,7-四羟基-4′-甲氧基二氢黄酮(5)、(2R,3R)-二氢槲皮素-7-甲醚(6)、槲皮素(7)、木犀草素(8)、圣草酚(9)、异半皮桉苷(10)、异槲皮苷(11)、3-甲氧基槲皮素(12)、熊果酸(13)、熊果酸内酯(14)、过氧麦角甾醇(15)、2-羟基-4,6-二甲氧基苯乙酮(16)、2,4-二羟基-6-甲氧基苯乙酮(17)、丹参素甲酯(18)。其中化合物10、1418为首次从艾纳香属植物中分离得到,化合物13为首次从植物艾纳香中分离得到。分别运用DPPH法及酪氨酸酶催化左旋多巴氧化速率法筛选化合物的体外抗氧化活性、酪氨酸酶抑制活性,发现化合物3~5、7~12、18有着较强的抗氧化性,化合物713141617有一定的酪氨酸酶抑制剂活性。

关键词: 艾纳香, 黄酮, 三萜类, 抗氧化活性, 酪氨酸酶抑制活性

Abstract:

To study the chemical constituents from the aerial parts of Blumea balsamifera DC. and their antioxidant and tyrosinase inhibitory activities. Eighteen compounds were isolated and identified from the aerial part of Blumea balsamifera DC. with various chromatographic techniques and spectroscopy methods,which were identified as 7,3′,4′-trimethylquercetin (1),4′,5-dihydroxy-3′,7-dimethoxyflavone (2),luteolin-7-methyl ether (3),rhamnetin (4),(2R,3R)-(+)-3,3′,5,7-tetrahydroxy-4′-methoxydihydroflavone (5),(2R,3R)-(+)-7′-O-methyldihydroquercetin (6),quercetin (7),luteolin (8),eriodictyol (9),isohemiphloin (10),isoquercetin (11),3-methoxyquercetin (12),ursolic acid (13),ursolic acid lactone (14),peroxyergosterol (15),2-hydroxy-4,6-dimethoxyacetophenone (16),2,4-dihydroxy-6-methoxyacetophenone (17),and methyl 3,4-dihydroxyphenyl lactate (18). Among them,compounds 10,14 and 18 were isolated from genus Blumea for the first time,and compound 13 was isolated from B. balsamifera for the first time. The antioxidant and anti-tyrosinase activities of these compounds were screened by using the DPPH free radical method and colorimetric method with L-tyrosine as substrat. The results showed that compounds 3-5,7-12 and 18 have strong antioxidant activities,and compounds 7,13,14,16,17 possessed certain tyrosinase inhibitory effects.

Key words: Blumea balsamifera, flavonoid, triterpenoids, antioxidant activity, tyrosinase inhibitory activity

中图分类号:  R914.4