关键词: 当归, 多糖, 分离, 纯化, 鉴定

Abstract: Angelica polysaccharide was extracted from Angelica by water extraction and ethanol precipitation.The obtained Angelica polysaccharide was deproteinized with Savage method combined with Papain method.It was then decolorized with H₂O₂ followed by dialysis.Finally five fractions were obtained by using DEAE-cellulose 52 column chromatography. The fivefractions were identified by Ultraviolet spectroscopy and SephadexG-100.The results showed that they were all single component.The five fractions were further analyzed byinfrared spectrometry,thin-layer chromatography and GC-MS.The results showed that the components of the five polysaccharide were all pectin polysaccharide.WASP-1 might be pyran ring Angelica polysaccharide compound;WASP-2 was β-type pyran ring Angelica polysaccharide compound;WASP-3 was β-type pyran ring and α-type D-pyran ring Angelica polysaccharide compound;WASP-4 was pyran ring Angelica polysaccharide compound;WASP-5 was β-typeAngelica polysaccharide compound,and might contain pyran ring;The GC-MS analysis results showed that sorbose,arabinose,glucose andgalactose were found in WASP-1,WASP-2 and WASP-3;Glucose and galactose were found in WASP-4;Glucose was found in WASP-5.

Key words: Angelica sinensis, polysaccharide, isolation, purification, property identification