NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 52-55.doi: 10.16333/j.1001-6880.2015.S.014

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Effect of Plant Ages on the Accumulation of Polyphenols from Bozhou Peony Paeonia lactiflora

CHEN Nai-dong1,2,3,ZHU Wang-sheng 1,CHEN Nai-fu1,YAO Hou-jun1, DING Huan-huan1,XIE Xiao-le1, LI Jun3*   

  1. 1 College of Biotechnology and Pharmaceutical Engineering,West Anhui University; 2 West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine,West Anhui University,Anhui Lu’an 237012,China; 3 Anhui Medical University,Anhui Hefei,230032,China
  • Online:2015-12-01 Published:2016-01-12

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Abstract: In order to explore the effects of cultivation ages on the accumulation of polyphenols in Bozhou Peony Paeonia lactiflora and provide references for the standardized cultivation and quality evaluation of Bozhou Peony,A HPLC-DAD method was established for analyzing the polyphenols in different years old Bozhou Peony Paeonia lactiflora. The separation was performed on a Lanbo service 4000 and waters Symmetry C18 column (4.6 mm×150 mm,5 μm) at 30 ℃ with a gradient elution of methanol and 1% acetic acid as mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was 280 nm.Five polyphenols were checked in Bozhou peony with different plant years while the contents and ratio of different polyphenols were obviously different.The accumulation patterns of different kinds of polyphenols were noticeably effected on by their cultivation ages.

Key words: Bozhou Peony Paeonia lactiflora, polyphenols, HPLC

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