Abstract: The aim of this study was to investigate the effects of 3,5,2′,4′-tetrahydroxych alcone (P40) on serum uric acid level,the mRNA expression level of hypoxanthineguanine phosphoribosyl transferase (HGPRT) of brain,5’-phosphoribosyl-1’-pyrophosphate synthetase (PRPS) and phosphoribosyl amidotransferase (PRPPAT) of liver in hyperuricemic mice. The hyperuricemic mice were induced by an intraperitoneal injection of uricase inhibitor potassium oxonate. All drugs were given intragastrically twice daily for five doses. Serum uric acid levels were determined at 1 h after the last dosage. The effects of P40 with different doses on HGPRT,PRPS and PRPPAT mRNA expression level were examined by the means of RT-PCR. The results showed serum uric acid levels in the model group were increased obviously (P<0.01). Compared with the model group,serum uric acid levels were decreased significantly in groups received P40 at the doses of 2,4,8 mg/kg (P<0.05 or P<0.01). P40 at doses of 2,4,8 mg/kg had no significant effect on the mRNA expression of HGPRT,PRPS and PRPPAT in hyperuricemic mice (P>0.05). Hence,P40 can reduce the serum uric acid levels in oxonate-induced hyperuricemic mice,but not inhibiting the mRNA expression of HGPRT in brain as well as PRPS and PRPPAT in liver under the experimental condition.

Key words: 3,5,2&prime, ,4&prime, -tetrahydroxychalcone, hyperuricemia, hypoxanthine guanine phosphoribosyl transferase, 5&prime, -phosphoribosyl-1&rsquo, -pyrophosphate synthetase, phosphoribosyl amidotransferase