Abstract: To explore the intervention effect of Huayu Tongluo traditional Chinese medicine on diabetic nephropathy (DN) and its effect on skeleton protein.Ten rats were randomly selected as the normal group (C group ) from 50 male Sprague-Dauley rats,the other 40 rats were injected with 1% streptozotocin (35 mg/kg) after fed high-fat and high-glucose diet for one month.The successful model rats (blood glucose≥16.7 mmol/L) were randomly divided into three groups:model group (M group),Huayu Tongluo traditional Chinese medicine group (Z group),Irbesartan group (I group).Each treatment group was given corresponding intervention,once a day for 16 weeks.At the end of the 16th week,24 h UTP,blood coagulation and platelet parameters were tested.The protein and mRNA expressions of α-actinin-4 and Synaptopodin in kidney tissue were examined by Western-blot and Real-time PCR methods.The results showed that 24 h UTP was significantly increased in other groups (P<0.01) compared with C group,and in two treatment groups it was also significantly improved (P<0.01) compared with M group,and it reduced significantly in Z group than that in I group (P<0.05).Similar results appeared that compared with C group,the expression of α-actinin-4 and Synaptopodin reduced in other groups (P<0.01),it was significantly higher in two treatment groups (P<0.01) than that in M group,and expression in Z group was significantly higher (P<0.05)than that in I group.The results indicated that Huayu Tongluo traditional Chinese medicine can reduce urinary protein in DN rats.The mechanism may be related to improve the high blood viscosity,hypercoagulable state and up regulating the expression of a-actinin-4 and synaptopodin.

Key words: diabetic nephropathy, Huayu-tongluo traditional Chinese medicine, urine protein, skeleton protein