Isolation and Identification of Secondary Metabolites from the  Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica

doi:10.16333/j.1001-6880.2016.11.008

Abstract

Abstract: Ten chemical constituents were isolated and purified from the solid culture of endophytic fungal strain Pestalotiopsis uvicola GMH31 isolated from Artemisia japonica by silica gel column chromatography,Sephadex-LH20 column chromatography and recrystallization.On the basis of spectral data and physicochemical properties,their structures were identified as thymine (1),uracil (2),luteolin (3),rutin (4),quercetin (5),vanillic acid (6),protocatechuic acid (7),kaempferol (8),genistein (9) and 4′,5,7-trihydroxyflavanone (10).All the compounds were isolated from P.uvicola for the first time,and compound 3~5 and 8~10 belong to flavonoids.Compound 8 showed better reversal effect on the ovarian cancer cell line A2780/Taxol.

Key words: Pestalotiopsis uvicola, endophytic fungus, secondary metabolite, Artemisia japonica

CLC Number:

R284.1

QIAN Yi-xin1,KANG Ji-chuan1,2*,HE Jun1,WANG Lu1,LEI Bang-xing1. Isolation and Identification of Secondary Metabolites from the Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(11): 1732-1735.

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Isolation and Identification of Secondary Metabolites from the Solid Culture of Endophytic Fungal Strain Pestalotiopsis uvicola GMH31 from Artemisia japonica

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