Abstract: The bottlenecks in natural drug discovery and development are cellular target identification.Affinity-based Protein Profiling (ABPP),exploiting protein-reactive functional group in many natural product structure (NPs),is a new technique used in identifying the drug targets of these NPs.This review article describes the current status of ABPP of this field and some examples according to structural skeletons of NPs are given.In addition,the advantages and disadvantages of probe molecules designed from NPs are discussed in detail.We also prospect the development of ABPP and other new techniques used to identify drug targets of NPs.

Key words: natural products, chemical proteomics, ABPP, target identification