The fingerprint of aloe from different origins was established,and the chromatographic separation was performed on a Phenomenex C₁₈ column (250 mm×4.6 mm,5μm),and a mixture of methannol-acetonitrile-0.3 % phosphoric acid water solution was selected as the mobile phase for gradient elution.The high-performance liquid chromatography (HPLC) fingerprint and chemical pattern recognition were studied to provide new method for quality control and evaluation of the different production areas of aloe.The results revealed that the HPLC fingerprint of aloe was calibrated with a total of 23 common peaks,and 6 common peaks could be identified using the references.The similarities of other medicinal materials was above 0.93 except for three batches of Guangxi medicinal materials and 12 batches of aloe could be divided into 3 groups by both cluster analysis and principal component analysis (PCA).Furthermore,the quality differences of aloe from different origins were mainly caused by 5 common peaks according to the results of orthogonal partial least squares discriminant analysis (OPLS-DA).Meanwhile,the contents of 6 components were determined by the same HPLC method.In brief,the establishment of HPLC fingerprint combined with the application of chemical pattern recognition could provide a simple and accurate method for the quality control and evaluation of aloe．