To study the secondary metabolites of endophytic fungus Letendraea helminthicola derived from Amomum villosum,eight compounds were isolated and purified from the culture of the fermentation by the silica gel,reverse phase gel silica,Sephadex LH-20 and high performance liquid chromatograohy (HPLC),their structures were identified by physical and chemical properties and spectroscopic data.Eight compounds including one new sesquiterpene 11-hydroxyl tricinonoic acid (1) and seven known compounds were isolated and identified as methyl 3-(3,5-di-ter-butyl-4-hydroxyphenyl) propionate (2),gibepyrone F (3),gibepyrone D (4),p-hydroxybenzaldehyde (5),p-methoxyphenylacetic acid (6),m-hydroxyphenylacetic acid (7),ergosterol (8).Compounds 2-8 were first isolated from the genus Letendraea.Compounds 1-5 were evaluated for their cytotoxic activities against human tumor cell lines by the SRB assay and their antibacterial activities against bacteria were evaluated by microdilution method.However,all the measurement compounds showed no antimicrobial and anticancer activities even at the concentration at 100 μg/mL.