Abstract: To investigate the effect of Laminaria japonica polysaccharide (LJPS) on the expression of calcitonin receptor-like receptor (CrlR) and the hypoglycemic effect on type 2 diabetes mellitus mice.Type 2 diabetes mellitus mouse model was established by feeding high fatty forage and injecting alloxan intraperitoneally in 40 healthy male mice and treated with LJPS orally.The level of fasting blood glucose (FBG) was detected by automatic blood glucose device.The expression of CrlR was determined by immunohistochemisty and Western blot,and the expression of CrlR mRNA was detected by RT-PCR.After treated with L.japonica, the serum level of FBG were decreased significantly compared with that of model group (P<0.05).In the liver and pancreas,the expression levels of CrlR mRNA and CrlR protein were significantly higher than those in model group (P<0.05).This experiment showed that LJPS played a hypoglycemic effect by promoting the expression of CrlR in liver and pancreatic tissue to lessen insulin resistance (IR).

Key words: Laminaria japonica polysaccharide, type 2 diabetes, amylin, calcitonin receptor-like receptor, mice