Abstract: A new ginsenoside Rg₁-bonded silica gel stationary phase with natural active ingredient was prepared by “one-pot” reaction method,using 1,6-diisocyanate as spacer arm.The characterization of new self-made ginsenoside Rg₁-bonded silica gel stationary was achieved by solid state NMR.An HPLC method for determination of six saponins,including notoginsenoside R₁,ginsenoside Rg₁, ginsenoside Re,ginsenoside Rb₁,ginsenoside Rb₃ and ginsenoside Rd in Xuesaitong was first developed by HPLC,using ginsenoside Rg₁-bonded silica gel stationary phase.Six components of saponins of Panax nototginseng could be simultaneously determined by ginsenoside Rg₁-bonded silica gel stationary phase,which will benefit for quality control.It’s a new idea for natural products analysis using ginsenoside Rg₁-bonded silica gel stationary phase.

Key words: ginsenoside Rg₁-bonded silica gel stationary phase, characterization of stationary phase, determination, saponin , Xuesaitong