Separation and Purification of Lectin from Morus alba L.and Its Antimicrobial Activity

doi:10.16333/j.1001-6880.2015.03.022

Abstract

Abstract: Morus alba lectin was firstly extracted with phosphate buffer and followed by a series of purification including ammonium sulfate graded precipitation.Further separation was carried out using Cellulose DE-52 chromatography,CM-Sepharose Fast Flow chromatography and Sephadex G-75sieve chromatography.The antimicrobial activity of M.alba lectin was finally tested using Oxford Cup method.The results showed that M.alba lectin displayed a single band detected with SDS polyacrylamide gel electrophoresis method and presented as 35.95 KD and 30.35 KD molecular weight.The lectin exhibited an inhibitory effect against Trichoderma viride, Alternaria alternata f.sp.mali,watermelon fusarium wilt and Phytophthora parasitica with antibacterial circle diameter of 20,30,15 mm and 18 mm,respectively.In summary, M.alba lectin exhibited an effective antimicrobial activity and therefore can be used as an useful natural lead compound for discovering and developing novel agrochemicals.

Key words: Morus alba lectin, antimicrobial activity, natural lead compound

CLC Number:

Q512

LIU Peng-ju1,FU Zhi-hui2,SUN Dong1,GUAN Ai-ying3,MA Di1*. Separation and Purification of Lectin from Morus alba L.and Its Antimicrobial Activity[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(3): 475-479.

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Separation and Purification of Lectin from Morus alba L.and Its Antimicrobial Activity

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