The secondary metabolites of Chaetomium funicola CIB-604 were investigated in this paper.Eight compounds were isolated from the solid fermented rice culture by silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative liquid chromatography.On the basis of ¹H NMR,¹³C NMR and ESI-MS spectroscopic data,their structures were identified as chaetoindicin A（1）,chaetoindicin B（2）,chaetoindicin C（3）,(22E,24R)-3β,5α,9α-trihydroxy-ergosta-7,22-dien-6-one（4）,(22E,24R)-5α,6α-epoxy-ergosta-8,22-dien-3β,7α-diol（5）,5α,6α-epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7α-diol（6）,ergosterol (7),and ergosterol peroxide (8).Compounds 1-8 were isolated from C. funicola for the first time. Compounds 1-6 showed no obvious inhibitory effect on Escherichia coli, Bacillus subtilis and Candida albicans at 100 μg/mL.Compound 4 exhibited significant cytotoxicities to SK-Hep-1,A549,HCT-116 and MCF-7 cells,while compound 2 showed moderate cytotoxicities against these cell lines.