NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (Suppl1): 80-83.doi: 10.16333/j.10016880.2016.S.020

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Determination of Antioxidant Active Site of Morchella angusticeps by HPLCDPPH

YOU Jinkun*,WU Surui,GAO Guanshi,YAN Ming,GUI Mingying   

  1. Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooporatives,Kunming 650221,China
  • Online:2016-06-30 Published:2017-03-02

Abstract: Online HPLCDPPH was used for determination of antioxidant active site from M.angusticeps.It mainly investigates the antioxidant activity of the aqueous extract、50% ethyl alcohol extract、95% ethyl alcohol extract was extracted by SPE,eluted with methanol,and chloroform extract.Based on this method,17 antioxidants have been found in M.angusticeps,among them,11 antioxidants have been found in aqueous extract、2 antioxidants have been found in 50% ethyl alcohol extract,and both of them showed high antioxidant activity.However,the results showed a minor constituent and not significant antioxidant activity in 95% ethyl alcohol extract and chloroform extract.M.angusticeps showed high antioxidant activity,the main components are polarity small molecular compounds,and are rich in aqueous extract.The antioxidants in M.angusticeps is well worth researching into.

Key words: HPLCDPPH, online detection, Morchella angusticeps, antioxidant activity

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