Abstract: To study the effects of Berberis diaphana Maxim on hypoglycemic and hypolipidemia activity in diabetic mice by using different preparations (vinegar treated and processing) and extraction methods (reflux,ultrasound,reflux and ultrasound).We set up several diabetic mice models of alloxan,streptozotocin (STZ),epinephrine,and high concentration glucose,in order to measure the glucose levels of the treatment and control groups before/after taking different groups of Berberis,and the values of TG,TC,HDLC and LDLC in the serum.We find that the extraction methods in three groups has no obvious effects on FBG of natural mice but lowering the blood glucose of alloxan mice obviously;the combination of reflux and ultrasound group in all treated Berberis has significant difference compared with dosage in no treated Berberis with decoction group (P<0.05);the vinegar treated Berberis with decoction,the high dosage group of alcohol treated Berberis with reflux,the vinegar treated Berberis with reflux,all ultrasound groups,and the combination of reflux and ultrasound groups all have obvious hypoglycemic effect on streptozotocin mice (P<0.05 or P<0.01);of reflux and ultrasound group with vinegar treated and alcohol treated in high dose has obvious hypoglycemic effects by high concentration glucose after 0.5,1 and 2 h (P<0.05 or P<0.01);the alcohol treated Berberis,the vinegar treated Berberis with reflux,the vinegar treated Berberis with ultrasound,and the high dosage groups of combination of reflux and ultrasound by no treated,vinegar treated,and alcohol treated Berberis all can lower the blood glucose of epinephrine hydrochloride mice obviously (P<0.05 or P<0.01);the vinegar treated Berberis with reflux,the combination of reflux and ultrasound by vinegar treated,and alcohol treated Berberis can lower the content of TG,TC,LDLC and rise the content of HDLC.The experiment shows that the combination of reflux and ultrasound by vinegar treated,and alcohol treated Berberishas better curative effects on hypoglycemic and hypolipidemia activity.

Key words: Berberis diaphana Maxim, processing, extraction method, blood glucose, blood lipid