NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (10): 1728-1731. doi: 10.16333/j.1001-6880.2015.10.008

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Isolation and Identification of Secondary Metabolites of Endophytic Fungi from Solanum tuberosum

YANG Man-si, WANG Wen-xiang, LI Long-gen, AI Hong-lian*, GUO Hua-chun*   

  1. School of Agriculture and Biological Technic,Yunnan Agricultural University,Kunming 650201,China
  • Online:2015-10-30 Published:2015-10-30

Abstract: This paper reported the isolation and identification of chemical components of the fermentation products of endophytic fungi E-19,isolated from Solanum tuberosum. This fungus was identified as Bipolaris eleusines on the basis of phylogenetic tree analysis of its 18S rDNA sequence.The secondary metabolites were isolated and purified using gel column chromatography,semi-preparative HPLC and Sephadex LH-20.Their structures were determined to be isosativenediol (1),dihydroprehelminthosporo (2),helminthosporol (3),drechslerines C (4),drechslerines A (5),secolongifolene diol (6) on basis of spectroscopic analysis and comparison with literatures.

Key words: Solanum tuberosum, endophytic fungus, Bipolaris eleusines;chemical constituent;structural identification

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