红芪、黄芪蜜炙前后配伍醋莪术含药血清对肺癌细胞增殖、迁移和侵袭的影响

doi:10.16333/j.1001-6880.2026.9.011

天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (9): 1972-1979.doi: 10.16333/j.1001-6880.2026.9.011 cstr: 32307.14.1001-6880.2026.9.011

红芪、黄芪蜜炙前后配伍醋莪术含药血清对肺癌细胞增殖、迁移和侵袭的影响

马定财 1,2,刘婷 1,2,杜欣霞 1,2, 巩文林 1,2,张育贵 2,辛二旦 1,2,边甜甜 1,李越峰 2∗

1甘肃中医药大学药学院;2 甘肃中医药大学科研实验中心,兰州730000

出版日期:2026-09-24 发布日期:2026-09-22
基金资助:
国家自然科学基金(82574743、82560778);2024 年甘肃省人才重点项目(2024RCXM77);2024 年甘肃省联合科研基金(24JRRA882)

Effects of containing serum from Hedysari Radix and Astragali Radix before and after honey-processing paired with vinegar-processed Curcumae Rhizoma on the proliferation,migration and invasion of lung cancer cells

MA Ding-cai1,2,LIU Ting1,2,DU Xin-xia1,2, GONG Wen-lin1,2,ZHANG Yu-gui2,XIN Er-dan1,2,BIAN Tian-tian1,LI Yue-feng2∗

1College of Pharmacy,Gansu University of Chinese Medicine; 2 Scientific Research and Experimental Center,Gansu University of Chinese Medicine,Lanzhou 730000,China

Online:2026-09-24 Published:2026-09-22

摘要/Abstract

摘要：

研究红芪(Hedysari Radix,HR)、黄芪(Astragali Radix,AR)蜜炙(honey processed,HP)前后配伍醋莪术(vinegar-processed Curcumae Rhizoma,VPCR)对A549 和NCI-H1299 细胞增殖、迁移和侵袭的影响。将49 只SD 雄性大鼠随机分为AR-VPCR、HPAR-VPCR、HR-VPCR、HPHR-VPCR、顺铂(cis-diaminodichloroplatinum,CDDP) + HPHR-VPCR 含药血清组、CDDP 组和空白组,连续灌胃7 d,采集血清;采用CCK-8 法筛选CDDP 抗A549 细胞的最佳浓度。集落实验检测细胞克隆能力,流式细胞术检测细胞周期,划痕实验检测细胞迁移能力,Transwell 小室实验检测细胞侵袭能力。结果发现,CDDP 干预A549 细胞和NCI-H1299 细胞的最佳浓度分别是20. 99、15. 25 mg/ L。AR-VPCR、HPARVPCR、HR-VPCR、HPHR-VPCR、CDDP、CDDP + HPHR-VPCR 均显著抑制A549 细胞和NCI-H1299 细胞克隆、迁移和侵袭(P < 0. 01)。在抑制细胞克隆方面,HR-VPCR 组优于AR-VPCR 组,HPHR-VPCR 优于HPAR-VPCR 组,HPHRVPCR 组抑制2 株细胞克隆的作用更明显。在抑制细胞迁移方面,HPHR-VPCR 联合CDDP 可增强抑制作用,HPARVPCR 强于AR-VPCR 组。AR-VPCR、HPAR-VPCR、HR-VPCR、HPHR-VPCR 将A549 细胞和NCI-H1299 细胞DNA 复制阻滞在G₁期,CDDP、CDDP + HPHR-VPCR 将A549 细胞DNA 复制阻滞在S 期。综上,AR-VPCR、HPAR-VPCR、 HR-VPCR、HPHR-VPCR、CDDP、CDDP + HPHR-VPCR 均可显著影响细胞周期,抑制A549 细胞和NCI-H1299 细胞克隆、迁移和侵袭。

关键词:

"> 炙红芪-醋莪术, 肺癌, 增殖, 迁移和侵袭

Abstract:

This study aims to investigate the effects of Hedysari Radix (HR),Astragali Radix (AR),and their combinations with vinegar-processed Curcumae Rhizoma (VPCR) before and after honey processing (HP) on the proliferation,migration, and invasion of A549 and NCI-H1299 cells. Forty-nine male SD rats were randomly divided into seven groups:AR-VPCR, HPAR-VPCR,HR-VPCR,HPHR-VPCR,cis-diaminodichloroplatinum(CDDP) + HPHR-VPCR containing serum,CDDP,and control group. Serum was collected after seven consecutive days of intragastric administration. The optimal concentration of CDDP against A549 cells was screened by the CCK-8 assay. Colony formation assay was used to detect cell clonogenicity,flow cytometry to analyze the cell cycle,wound-healing assay to evaluate cell migration,and Transwell chamber assay to measure cell invasion. The results showed that the optimal concentrations of CDDP for A549 and NCI-H1299 cells were 20. 99 and 15. 25 mg/ L,respectively. AR-VPCR,HPAR-VPCR,HR-VPCR,HPHR-VPCR,CDDP,and CDDP + HPHR-VPCR all significantly inhibited colony formation,migration,and invasion of A549 and NCI-H1299 cells (P < 0. 01). In terms of colony inhibition,the HR-VPCR group was superior to the AR-VPCR group,and the HPHR-VPCR group was superior to the HPARVPCR group,with HPHR-VPCR showing stronger inhibitory effects on both cell lines. For migration inhibition,the combination of HPHR-VPCR and CDDP exerted a synergistic inhibitory effect,and HPAR-VPCR was more effective than AR-VPCR. AR-VPCR,HPAR-VPCR,HR-VPCR,and HPHR-VPCR arrested DNA replication of A549 and NCI-H1299 cells in the G1 phase,while CDDP and CDDP + HPHR-VPCR blocked DNA replication of A549 cells in the S phase. In conclusion,ARVPCR,HPAR-VPCR,HR-VPCR,HPHR-VPCR,CDDP,and CDDP + HPHR-VPCR can significantly affect the cell cycle and inhibit colony formation,migration,and invasion of A549 and NCI-H1299 cells.

Key words:

"> honey-processed Hedysari Radix-vinegar-processed Curcumae Rhizoma, lung cancer, proliferation, migration and invasion

中图分类号:

R283. 1','1');return false;" target="_blank"> "> R283. 1 R285. 5

马定财, 刘婷, 杜欣霞, 巩文林, 张育贵, 辛二旦, 边甜甜, 李越峰. 红芪、黄芪蜜炙前后配伍醋莪术含药血清对肺癌细胞增殖、迁移和侵袭的影响[J]. 天然产物研究与开发, 2026, 38(9): 1972-1979.

MA Ding-cai, LIU Ting, DU Xin-xia, GONG Wen-lin, ZHANG Yu-gui, XIN Er-dan, BIAN Tian-tian, LI Yue-feng. Effects of containing serum from Hedysari Radix and Astragali Radix before and after honey-processing paired with vinegar-processed Curcumae Rhizoma on the proliferation,migration and invasion of lung cancer cells[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(9): 1972-1979.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: http://www.trcw.ac.cn/CN/10.16333/j.1001-6880.2026.9.011

http://www.trcw.ac.cn/CN/Y2026/V38/I9/1972

[1]	王江瑞, 蔡蓓蕾, 曾钦, 赵江怡, 王钧, 钱勇, 赵建权. 基于UPLC-Q-TOF-MS/MS的火把花根药渣中化学成分研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 79-92.
[2]	史芯怡, 杨天云, 周广之, 蒋玉虹, 农文龙, 甘斌, 李锦锋, 黄伟文. 蔗糖碳球负载金花茶花托有效成分及其抗菌性能研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 93-99.
[3]	杨佳倩, 阳红燕, 张晓梅. 虫生真菌Metapochonia suchlasporia Y9次生代谢产物研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 100-105.
[4]	张梦潇, 周传顺, 程鸣, 唐敏. 蒸制黄精中麻味物质的去除机制与感官特性分析[J]. 天然产物研究与开发, 2026, 38(增刊1): 106-118.
[5]	王楠, 范丽萍, 吕佳昊, 沈颖, 叶继锋. 肉桂醛/魔芋葡甘聚糖/果胶复合膜的制备及性能研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 119-127.
[6]	王霈菲, 傅竹生, 朱跃进, 傅秀花, 蒋玉兰, 潘俊娴, 韦阳, 吕杨俊. 不同干燥方式对速溶茶挥发性物质的影响[J]. 天然产物研究与开发, 2026, 38(增刊1): 128-138.
[7]	何靖容, 商亚芳, 白文博, 赵美婷, 李芊, 张婉, 张立军. 大马士革玫瑰废水多糖的理化性质与生物活性研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 139-147.
[8]	魏代静, 赵书林, 陈代宏, 王怡, 刘军, 刘莉, 杨旭, 南国辉. 滇黄精品质多指标评价及川滇产区地域关联性分析[J]. 天然产物研究与开发, 2026, 38(增刊1): 148-157.
[9]	闫淑萍, 王建成, 张薛勤, 王玥, 史可, 邢岩, 张丽梅. 超临界CO₂萃取青花椒油胺的成分分析及舒缓抗皱作用研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 158-164.
[10]	乌日罕, 小芳, 邢煜舒, 高钰思. 高优-巴沙嘎通过PI3K-AKT信号通路抑制乳腺癌细胞迁移活性的机制研究[J]. 天然产物研究与开发, 2026, 38(增刊1): 165-172.
[11]	刘坤, 刘一凡, 陈立成, 王纯, 孔泽娟, 赵庆生, 程华, 王军朋. 基于UHPLC-Q-Exactive-Orbitrap-MS与网络药理学探究桑树桑黄乙醇提取物抗衰老的物质基础和作用机制[J]. 天然产物研究与开发, 2026, 38(增刊1): 173-184.
[12]	高雅倩, 刘嘉颖, 梁逸恒, 杜志云. 基于网络药理学与动物模型探究黄芪-葛根对噪声性听力损失的修复作用[J]. 天然产物研究与开发, 2026, 38(增刊1): 185-191.
[13]	郑宏斌, 王鑫, 符惠娴, 曾亚红, 陈国德, 苟志辉. 沉香中倍半萜类化合物与2-(2-苯乙基)色酮类化合物研究进展[J]. 天然产物研究与开发, 2026, 38(增刊1): 192-211.
[14]	李林, 张跃朋, 覃章辉, 刘华英, 南占东, 殷红清. 菊花精油的提取工艺、化学成分与生物活性研究进展[J]. 天然产物研究与开发, 2026, 38(增刊1): 212-217.
[15]	高瑞萍, 涂鸿阳, 唐维, 梁明艳, 王勇德, 李守宝, 吴振. 炮制加工和提取对天麻酚苷类成分影响的研究进展[J]. 天然产物研究与开发, 2026, 38(增刊1): 218-229.

红芪、黄芪蜜炙前后配伍醋莪术含药血清对肺癌细胞增殖、迁移和侵袭的影响

Effects of containing serum from Hedysari Radix and Astragali Radix before and after honey-processing paired with vinegar-processed Curcumae Rhizoma on the proliferation,migration and invasion of lung cancer cells

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价