天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 968-979.doi: 10.16333/j.1001-6880.2020.6.009

• 研究论文 • 上一篇    下一篇

鲜鱼腥草UPLC特征图谱及5种指标成分含量测定研究

徐杰,曾昭君,邓李红,魏梅,黄梦婷,刘燎原*,程学仁   

  1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室,佛山 528244
  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    广东省科技计划(2018B030323004);广东特支计划科技创业领军人才项目(2017TY04R197)

Study on the determination of five index components and characteristic chromatogram of fresh Houttuynia cordata by UPLC

XU Jie,ZENG Zhao-jun,DENG Li-hong,WEI Mei,HUANG Meng-ting,LIU Liao-yuan*,CHENG Xue-ren   

  1. Guangdong Yifang Pharmaceutical Co.,Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,China

  • Online:2020-06-28 Published:2020-07-06

摘要:

为建立鲜鱼腥草药材UPLC特征图谱及5种指标成分含量同时测定的方法,本研究采用UPLC技术,以乙腈∶0.1%磷酸水溶液为流动相梯度洗脱,流速0.3 mL/min,检测波长分别为254和326 nm,同时测定不同产地16批鲜鱼腥草药材中新绿原酸、绿原酸、隐绿原酸、金丝桃苷、槲皮苷含量及特征图谱,采用超高效液相色谱-质谱(UPLC-MS)对共有峰进行了指认。实验结果显示,本研究建立的特征图谱及含量测定方法符合方法学要求,5种指标成分的含量均有不同程度的差异;特征图谱共标定了7个共有峰,指认了其中5个峰;不同产地鲜鱼腥草药材与对照特征图谱的相似度在0.890~0.999之间;聚类分析和主成分分析均可将16批鲜鱼腥草分为3类,不同类别间质量存在差异;主成分综合得分结果表明浙江金华种植的鲜鱼腥草质量最优,其次是四川彭州、广汉、绵阳等地。结果表明本研究建立的UPLC特征图谱及5种指标成分含量同时测定方法可用于鲜鱼腥草药材质量的综合评价。

关键词: 鲜鱼腥草, UPLC, 特征图谱, 含量测定, 聚类分析, 主成分分析

Abstract:

To establish a method for simultaneous analysis on five index components determination and characteristic chromatogram of fresh Houttuynia cordata by ultra performance liquid chromatography(UPLC).The UPLC method was carried out by gradient elution with aqueous phosphoric acid (0.1%).The flow rate was 0.3 mL/min.The detection wavelength was at 254 nm and 326 nm.The contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,hyperoside and quercetin of 16 batches of fresh H. cordata from different origins and the characteristics chromatogram were analyzed.The common peaks were identified by ultra performance liquid chromatography tandem-mass spectrometry(UPLC-MS).The methods for characteristic chromatogram analysis and compound determination were in agreement with methodological requirements.There are differences at different degrees in the contents of five indicative compounds;The common characteristic chromatogram of fresh H. cordata were completed with seven recognizable peaks and five of them determined;The similarity between the 16 batches of fresh H. cordata and the control characteristic chromatography was between 0.890 and 0.999.Both cluster analysis and principal component analysis can classify 16 batches of fresh H. cordata in three categories,and the quality of different categories is different.The results of main component comprehensive scores indicate that the quality of fresh H. cordata planted in Zhejiang Jinhua is the best,followed by Pengzhou,Guanghan,Mianyang and other places.The established UPLC characteristic chromatogram and simultaneous analysis on five index components determination methods can be used for the comprehensive quality evaluation of fresh H. cordata.

Key words: fresh Houttuynia cordata, UPLC, characteristic chromatogram, content determination, cluster analysis, principal component analysis

中图分类号:  R932