天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (2): 268-274.doi: 10.16333/j.1001-6880.2021.2.011

• 开发研究 • 上一篇    下一篇

2-羟基-3-甲基蒽醌抑制结直肠癌的作用及机制研究

谭佳妮1,2，刘文豪1,2，沈卫星1,2，孙东东1,2*，程海波1,2*   

  1. 1南京中医药大学转化医学研究中心 国家中医药管理局名医验方评价与转化重点实验室 江苏省抗肿瘤验方与产业化工程实验室；
    2江苏省中医药防治肿瘤协同创新中心，南京 210023
  • 出版日期:2021-02-28 发布日期:2021-03-04
  • 基金资助:
    国家自然科学基金（81930117，81804073，82074318）；江苏高校优势学科建设工程项目（苏政办发〔2018〕87号）

The anti-proliferative activity and mechanism exploration of 2-hydroxyl group-3-tectoquinone on colon cancer cells

TAN Jia-ni1,2,LIU Wen-hao1,2,SHEN Wei-xing1,2,SUN Dong-dong1,2*,CHENG Hai-bo1,2*   

  1. 1 Translational Medicine Center of Nanjing University of Chinese Medicine,Key Laboratory of Famous Doctors' Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine, Provincial Lab of Anticarcinoma Proved Recipe Research and Engineering Industrialization;2 TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Province,Nanjing 210023,China
  • Online:2021-02-28 Published:2021-03-04

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摘要： 为观察2-羟基-3-甲基蒽醌（HMA）对结直肠癌细胞增殖的影响并探讨其作用机制，本研究采用CCK8法检测HMA对结直肠癌细胞增殖的影响；Heochst-33343/PI染色检测细胞凋亡情况；同时检测细胞内ROS及GSH含量变化并应用Western blot检测细胞凋亡相关蛋白及Keap-1/Nrf-2/ARE信号途径相关蛋白的表达。实验结果显示，HMA给予结直肠癌细胞后，细胞内ROS含量升高，GSH含量减少；HMA通过抑制Keap-1/Nrf-2/HO-1通路，诱导细胞发生凋亡。综上表明HMA具有抑制结肠癌细胞增殖的作用，其机制可能与破坏细胞内氧化还原平衡，抑制Keap-1/Nrf-2/HO-1通路有关。

关键词: HMA, 结直肠癌, 氧化应激, Keap-1/Nrf-2/HO-1通路

Abstract:

To explore the anti-tumor effect and the possible mechanisms of HMA against colon cancer cells.The CCK8 methods was used to assay the anti-proliferative activity of HMA.The level of intracellular ROS and GSH was measured by commercial kit.Western blot assay was used to detected the protein expression change of Keap-1/Nrf-2/ARE signaling pathway.The results showed that HMA could significantly inhibit the proliferation of colon cancer cells (P<0.05).Compared to the control group,HMA increased the level of ROS while decreased the GSH content,and inhibited the Keap-1/Nrf-2/HO-1 pathway signaling.In conclusion,HMA could significantly inhibit the proliferation of colon cancer cells by disturbing the redox balance,which was related to inhibiting the Keap-1/Nrf-2/HO-1 signaling pathway.

Key words: HMA, colorectal cancer, oxidative stress, Keap-1/Nrf-2/HO-1 signaling pathway

中图分类号:  R285.5

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