天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (5): 773-778.doi: 10.16333/j.1001-6880.2021.5.008

• 研究简报 • 上一篇    下一篇

春砂仁内生真菌Letendraea helminthicola A696的次级代谢产物研究

张捷1,2,刘洪新2,刘昭明2,李赛妮2,陈玉婵2,章卫民2*,郭波红1*   

  1. 1广东药科大学药学院,广州 510006;2广东省科学院微生物研究所 省部共建华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室 广东省微生物应用新技术公共实验室,广州 510070

  • 出版日期:2021-05-28 发布日期:2021-06-01
  • 基金资助:
    国家自然科学基金(31699271);广东省科技计划(2107 A020211023)

Secondary metabolites of endophytic fungus Letendraea helminthicola derived from Amomum villosum

ZHANG Jie1,2,LIU Hong-xin2,LIU Zhao-ming2,LI Sai-ni2,CHEN Yu-chan2,ZHANG Wei-min2 *,GUO Bo-hong1 *   

  1. 1School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;2 State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China

  • Online:2021-05-28 Published:2021-06-01

摘要:

研究春砂仁内生真菌Letendraea helminthicola A696的次级代谢产物,采用硅胶柱色谱、ODS反相柱色谱、凝胶柱色谱及高效液相色谱等方法从该菌株的发酵液中分离得到8个化合物,通过理化性质及谱学数据分析,分别鉴定为11-hydroxyl tricinonoic acid(1)、3-(3,5-二叔丁基-4-羟基苯基)丙酸甲酯(2)、gibepyrone F(3)、gibepyrone D(4)、对羟基苯甲醛(5)、对甲氧基苯乙酸(6)、间羟基苯乙酸(7)、麦角甾醇(8),其中化合物1为新化合物,化合物2~8均为首次从Letendraea属真菌中分离得到。选取化合物1~5进行了抗菌和抗肿瘤活性试验,结果表明化合物1~5在100 μg/mL浓度下均无抗菌和抗肿瘤活性。

关键词: 内生真菌, 春砂仁, Letendraea helminthicola, 次级代谢产物

Abstract:

To study the secondary metabolites of endophytic fungus Letendraea helminthicola derived from Amomum villosum,eight compounds were isolated and purified from the culture of the fermentation by the silica gel,reverse phase gel silica,Sephadex LH-20 and high performance liquid chromatograohy (HPLC),their structures were identified by physical and chemical properties and spectroscopic data.Eight compounds including one new sesquiterpene 11-hydroxyl tricinonoic acid (1) and seven known compounds were isolated and identified as methyl 3-(3,5-di-ter-butyl-4-hydroxyphenyl) propionate (2),gibepyrone F (3),gibepyrone D (4),p-hydroxybenzaldehyde (5),p-methoxyphenylacetic acid (6),m-hydroxyphenylacetic acid (7),ergosterol (8).Compounds 2-8 were first isolated from the genus Letendraea.Compounds 1-5 were evaluated for their cytotoxic activities against human tumor cell lines by the SRB assay and their antibacterial activities against bacteria were evaluated by microdilution method.However,all the measurement compounds showed no antimicrobial and anticancer activities even at the concentration at 100 μg/mL.

Key words: endophytic fungus, Amomum villosum, Letendraea helminthicola, secondary metabolities

中图分类号:  R914.4