天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (6): 943-950.doi: 10.16333/j.1001-6880.2021.6.006

• 研究简报 • 上一篇    下一篇

朴树内生镰刀菌HU0174抗真菌次生代谢产物研究

李晓洁1,李丰名2,徐良雄2,李玲1*,彭永宏1,2*   

  1. 1华南师范大学生命科学学院,广州 510631;2惠州学院生命科学学院,惠州 516007
  • 出版日期:2021-06-28 发布日期:2021-07-01
  • 基金资助:
    国家自然科学基金(31772032);惠州学院自然科学研究项目(20170406122437260)

Antifungal secondary metabolites of endophytic Fusarium sp. HU0174 from Celtis sinensis

LI Xiao-jie1,LI Feng-ming2,XU Liang-xiong2,LI Ling1 *,PENG Yong-hong1,2 *   

  1. 1School of Life Sciences,South China Normal University,Guangzhou 510631,China;2School of Life Sciences,Huizhou University,Huizhou 516007,China

  • Online:2021-06-28 Published:2021-07-01

摘要: 通过硅胶柱层析、大孔吸附树脂柱层析和高效液相制备等色谱技术从一株朴树内生镰刀菌Fusarium sp.HU0174的大米发酵物中分离得到一个新的环酯肽:acuminatum D(1)和6个已知化合物:acuminatums A~C(2~4)、白僵菌素(5)、白僵菌酮(6)和吲哚-3-羧酸(7),运用NMR和MS等谱学方法鉴定了它们的化学结构。采用滤纸片琼脂扩散法测试表明环酯肽类化合物1~4对柑橘绿霉和新月弯孢霉两株植物致病真菌具有明显的体外抑制活性,初步阐明了镰刀菌HU0174的抗真菌活性物质基础。


关键词: 内生镰刀菌HU0174, 代谢产物, 环酯肽, 抗真菌活性

Abstract:

Antifungal secondary metabolites of the culture of an endophytic fungus,Fusarium sp.HU0174,from Celtis sinensis were investigated.A new cyclic depsipeptide acuminatum D (1),along with six known compounds acuminatums A-C (2-4),beauvericin (5),bassiatin (6) and indole-3-carboxylic acid (7),were isolated by silica gel column chromatography,macroporous adsorbent resin column chromatography and high-performance liquid chromatography.Their structures were determinated by means of NMR and mass spectra.Compounds 1-4 exhibited significant antifungal activities against the pathogenic Penicillium digitatum and Curvularia lunata by agar diffusion method.

Key words: endophytic Fusarium sp.HU0174, secondary metabolite, cyclic depsipeptide, antifungal activity

中图分类号:  O629.5