Ultra high performance liquid chromatography (UPLC) was used to establish the fingerprint of Curcumae Longae Rhizoma standard decoction and determine the contents of four active ingredients simultaneously to provide a basis for the formulation of quality standard.The fingerprints of Curcumae Longae Rhizoma standard decoction were established,and the common fingerprint peaks were identified by mass spectrometry analysis and comparison with reference materials.The fingerprints of 16 batches of samples were further processed and analyzed by chemometrics.At the same time,the contents of curcumin,noroxycurcumin ,bisdemethoxycurcumin and ferulic acid in the standard decoction were determined.Results showed that there were 10 common peaks in the fingerprints and 8 of them were identified.Hierarchical cluster analysis (HCA) and principal component analysis (PCA) classified 16 batches of samples into two categories,and six differential markers were found by orthogonal partial least squares discriminant analysis (OPLS-DA).The content determination results showed that the content of curcumin,noroxycurcumin,bisdemethoxycurcumin and ferulic acid in the standard decoction prepared from the raw material of in Dali,Yunnan Province was higher than that of the samples in Yibin and Leshan,Sichuan Province.The method is accurate and reliable,and can provide reference for the quality evaluation of Curcumae Longae Rhizoma standard decoction.