Abstract: Ultra-high performance liquid chromatography-four-stage rod-time-of-flight mass spectrometry (UHPLC-TOF-MS) was used to analyze the mass spectrometric decomposition and metabolic transformation of eupalinolide F in rats.Phenomenex Luna-C₁₈ (2 mm×100 mm,3 μm) column was used.The mobile phase was acetonitrile-0.1% formic acid aqueous solution,gradient elution,flow rate was 0.2 mL/min,column temperature was 30 °C,injection volume was 2 μL.The dynamic background subtraction trigger information correlation acquisition was used for MS/MS data acquisition.The metabolites were analyzed and identified by combining PeakView and MetabolitePilot software.Eupalinolide F undergoes phase I metabolism such as hydrolysis,oxidation,reduction,methylation,etc.,through the combination of glucuronidation,sulfuric acid esterification and glutathione II and multiple biotransformation pathways,formed a series of 55 related metabolites.The method is highly efficient and sensitive,and clarifies the metabolic transformation process of the eupalinolide F in rats,and provides scientific reference for the traditional Chinese medicine Eupatorium lindleyanum drug-effect material basis,new drug research and development and clinical application.

Key words: eupalinolide F, metabolites, UPLC-Q-TOF-MS, rats