Abstract: Four compounds were yielded by a microbial co-culture of the gorgonian-associated fungus Aspergillus sclertoiorum and Penicillium citrinum, including penicillic acid(1),5,6-dihydropenicillic acid(2),6-dihydro-6-hydroxy penicillic acid(3)and 2-hydroxy-3,6-dimethylbenzoic acid(4).The structures of them were elucidated by the analysis of NMR data and comparison with literatures.HPLC was applied for the analysis of the production of 1-4 and they were enhanced in different level in the co-culture compared with A.sclertoiorum cultured individually.Compound 4 was obtained from marine fungus for the first time.

Key words: Gorgonian-associated fungus, Aspergillus sclertoiorum, Penicillium citrinum, co-culture, secondary metabolites