天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (11): 1809-1817.doi: 10.16333/j.1001-6880.2021.11.001

• 研究论文 •    下一篇

竹叶花椒黄酮类成分含量及抗氧化活性相关性分析

杨悦,冯靖雯,陈鸿平,刘友平*   

  1. 成都中医药大学药学院 西南特色中药资源国家重点实验室,成都 611137
  • 出版日期:2021-11-28 发布日期:2021-12-02
  • 基金资助:
    国重·双一流·中药质量保障提升科研创新团(030041 011)

Correlation analysis of flavonoids content and antioxidant activity of Zanthoxylum armatum DC.

YANG Yue,FENG Jing-wen,CHEN Hong-ping,LIU You-ping*   

  1. State Key Laboratory of Southwest Characteristic Traditional Chinese Medicine Resources, School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

  • Online:2021-11-28 Published:2021-12-02

摘要: 为比较不同产地竹叶花椒黄酮类成分含量及抗氧化活性差异,探究黄酮成分与抗氧化活性相关性,以21个产地的竹叶花椒为材料,建立HPLC同时测定4种黄酮类成分含量(芦丁、金丝桃苷、异槲皮苷、槲皮苷)的方法,采用分光光度法测定总黄酮组分含量,采用DPPH、ABTS自由基清除试验评价其抗氧化活性,并结合皮尔逊相关分析探究黄酮类成分与抗氧化活性相关性。建立的方法简便、准确,可用于竹叶花椒中4种黄酮类成分的含量测定;不同产地竹叶花椒中黄酮类成分及抗氧化活性存在明显差异;相关性分析结果表明:总黄酮和芦丁与DPPH、ABTS清除率的EC50均呈显著负相关,因此,总黄酮与芦丁可作为评价竹叶花椒抗氧化能力的指标。本研究为竹叶花椒抗氧化活性研究提供实验依据,为其大健康衍生品开发提供实验依据。


关键词: 竹叶花椒, 产地, 黄酮, 抗氧化, 皮尔逊相关性分析

Abstract:

To compare the content of flavonoids and the antioxidant activity difference of Zanthoxylum armatum DC. from different places of production,the correlation between flavonoids and antioxidant activity is explored.Taking Z. armatum from 21 producing areas as the materials,this paper establishes a method to simultaneously determine the contents of four types of flavonoids (inculding rutinum,hyperoside,isoquercitrin,quercitrin) with HPLC,test the content of total flavonoids by adopting spectrophotometry,evaluate its antioxidant activity through DPPH and ABTS free radical scavenging tests,and analyzes and explores the correlation between flavonoids and antioxidant activity combined with product-moment coefficient of correlation.The established method is simple and accurate which can be used in testing the contents of four types of flavonoids in Z. armatum;there are obvious differences in the content of flavonoids and the antioxidant activity of Z. armatum from different places;according to the correlation analysis results,it can be seen that the total flavonoids and rutinum are significantly negative to the EC50 of both DPPH and ABTS clearance.Therefore,total flavonoids and rutinum can be used as indicators to evaluate the antioxidant capability of Z. armatum.This study provides an experimental basis for the antioxidant activity research of Z. armatum and a reference for the development of its health derivatives.

Key words: Zanthoxylum armatum DC., producing areas, flavonoids, antioxidant activity, Pearson correlation analysis

中图分类号:  R284