天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (12): 2097-2103.doi: 10.16333/j.1001-6880.2018.12.010

• 研究论文 • 上一篇    下一篇

荭草花有效组分在人肠道菌群中体外代谢特征分析

陈浩1,3，王昌权1,3，夏涛3，李月婷1，王永林1，黄勇1，李勇军2，郑林1*   

  1. 1贵州医科大学贵州省药物制剂重点实验室/ 药用植物功效与利用国家重点实验室；2贵州医科大学民族药与中药开发应用教育部工程研究中心；3贵州医科大学药学院，贵阳 550004
  • 出版日期:2019-01-02 发布日期:2019-01-02
  • 基金资助:

    国家自然科学基金（81360664，81560630，81660712）；贵州省科技计划(黔科合平台人才［2017］5601,［2016］5613\5677)；贵阳市科研创新团队项目（筑科合同［2017］30-29号）

Analysis of in Vitro Metabolic Characteristics of Effective Components of Polygonum orientale Flower in Human Intestinal Flora

CHEN Hao1,3,WANG Chang-quan1,3,XIA Tao3,LI Yue-ting1,WANG Yong-lin1,HUANG Yong1,LI Yong-jun2,ZHENG Lin1*   

  1. 1Guizhou Provincial Key Laboratory of Pharmaceutics/ State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;2Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education),Guizhou Medical University; 3School of Pharmacy,Guizhou Medical University,Guiyang 550004,China
  • Online:2019-01-02 Published:2019-01-02

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摘要： 为考察荭草花有效组分在离体人肠道菌群中的代谢特征。通过在离体培养的人肠道菌液中，加入荭草花有效组分，并在厌氧环境下进行培养，孵育的代谢产物采用超高效液相串联电喷雾飞行时间质谱仪进行检测。运用Metabolite ToolsTM分析平台对样品中的代谢产物进行预测分析，结合Mass Defect Filter 技术并运用 Data Analysis中的Smart Formula 功能推测代谢产物化学式。结果表明从药物-肠菌孵育液中共检测出14个代谢产物，主要包括N-p-香豆酰酪胺，N-trans-对羟基苯乙基阿魏酰胺的氢化、氢化羟基化、甲基化羟基化代谢产物，山柰酚的双脱氧、C2-C3双键还原、脱氧氢化代谢产物，槲皮素的羟基化、O-C2键开环裂解脱氧化、C2-C3双键还原O-C2键开环裂解代谢产物，儿茶素的氢化代谢产物和没食子酸的脱羧、甲基化代谢产物。上述结果表明荭草花有效组分在人离体肠道菌群中主要发生还原、氧化、水解Ⅰ相反应和甲基化Ⅱ相反应，为进一步研究其在体内的作用机制提供实验依据。

关键词: 荭草花有效组分, 代谢, 肠道菌群, UPLC-Q-TOF/MS

Abstract: In order to investigate the metabolic characteristics of the effective components of Polygonum orientale flower in human intestinal flora.The effective components of Polygonum orientale flower were added to in vitro culture of human intestinal bacteria and incubated in anaerobic environment.The metabolites were detected by UPLC-Q-TOF/MS.The metabolites in the samples were predicted and analyzed by using Metabolite ToolsTM analysis platform and the chemical formula of metabolites was deduced by using the Smart Formula function in Data Analysis combined with Mass Defect Filter technology.A total of 14 metabolites were detected from the drug-enteroinoculum solution,including N-p-coumaroyl tyramide,hydrogenation of N-trans-p-hydroxyphenethyl ferulamide,hydrogenation hydroxylation,and methylation.Hydroxylated metabolites,dodeoxylation of kaempferol,reduction of C2-C3 double bonds,deoxygenation metabolites,hydroxylation of quercetin,ring-opening cleavage and deoxidation of O-C2 bonds,and C2-C3 double bond reduction of O-C2 bonds Ring-opening pyrolysis metabolites,hydrogenated metabolites of catechins,and decarboxylated,methylated metabolites of gallic acid.The effective components of Polygonum orientale flower are mainly reduced,oxidized,hydrolyzed I phase reaction and methylated phase II reaction in isolated human intestinal flora,which will provide reference for further study of its mechanism in vivo.

Key words: Polygonum orientale flower effective components, metabolism, intestinal flora, UPLC-MS/MS

中图分类号: 

R285.1 R969.1

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