In this paper,six kinds of traditional Chinese medicine:Herba Moslae,Pseudobulbus Cremastrae Seu Pleiones,Radix Angelicae Pubescentis,Rhizoma Sparganii,Catechu,Radix Polygalae were extracted,and the activity of the extract was verified by inhibiting hyaluronidase activity,scavenging free radicals,and inhibiting histamine release.And it was added to the emulsion formula to evaluate the efficacy of soothing.The results showed that the inhibition rate of 6% prescription extract on hyaluronidase was 80.06%,and the scavenging rates on Superoxide anion radical,Hydroxyl radical,and DPPH radical were more than 70%.The histamine release rate of 200 μg/mL group extract was 52%,which indicated that the group extract had certain inhibition or scavenging effect on hyaluronidase,histamine release and three kinds of free radicals.The results of human efficacy evaluation showed that the emulsion with compound extract could significantly reduce the skin heme (P＜0.01),reduce the skin water loss (P＜0.01),and increase the skin water content (P＜0.01).This shows that the emulsion can effectively relieve skin allergic symptoms and enhance skin barrier function.